Full Clone List
List of Entry and Destination Vectors
Here is a list of the constructs in the Tol2kit. Click on any construct name (second column) to see more information. 12/18/07: Clone pages now include annotated sequence in Genbank format.
Here are insert sequences for entry clones, and 5' and 3' ends for destination clones, along with a description of how to assemble predicted sequences for expression clones.
Constructs in the Tol2kit v1.2 (Nov 2007)
| # | Name | Insert | Size (bp) | Made by | ver | MTA? |
|---|---|---|---|---|---|---|
| 5' entry clones, attL4-R1 (kan resistant) | ||||||
| 299 | p5E-bactin2 | 5.3 kb beta-actin promoter (ubiquitous) | 7950 | KMK | 1.0 | |
| 380 | p5E-h2afx | 1 kb histone2A-X promoter (quasi-ubiquitous) | 3604 | KMK | 1.0 | |
| 382 | p5E-CMV/SP6 | 1 kb CMV/SP6 cassette from pCS2+ | 3704 | KMK | 1.0 | |
| 222 | p5E-hsp70l | 1.5 kb hsp70l promoter for heat-shock induction | 4163 | BM | 1.0 | |
| 327 | p5E-UAS | 10x UAS element and basal promoter for Gal4 response | 3127 | DSC | 1.0 | |
| 228 | p5E-MCS | multiple-cloning site from pBluescript | 2810 | BM | 1.0 | |
| 381 | p5E-Fse-Asc | restriction sites for 8-cutters FseI and AscI | 2663 | EF | 1.0 | |
| Middle Entry Clones, attL1-L2 (kan resistant) | ||||||
| 383 | pME-EGFP | EGFP | 3327 | KMK | 1.0 | |
| 384 | pME-EGFPCAAX | membrane-localized (prenylated) EGFP; fused to 21 aa of H-ras | 3345 | KMK | 1.0 | |
| 385 | pME-nlsEGFP | nuclear-localized EGFP | 3342 | KMK | 1.0 | |
| 386 | pME-mCherry | monomeric red fluorophore mCherry | 3261 | KMK | 1.0 | |
| 232 | pME-mCherryCAAX H80D** | prenylated mCherry (deleterious H80D mutation; superseded by 450) | 3321 | KMK | 1.0 | |
| 233 | pME-nlsmCherry | nuclear-localized mCherry | 3288 | KMK | 1.0 | |
| 234 | pME-H2AmCherry | mCherry fused to zebrafish histone H2A.F/Z | 3651 | KMK | 1.0 | |
| 387 | pME-Gal4VP16 | Gal4 DNA binding domain fused to VP16 transactivation domain | 3204 | EF | 1.0 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 450 | pME-mCherryCAAX** | prenylated mCherry (some preps contam w/ H80D, superseded by #550) | 3321 | KMK/SYC | 1.2 | |
| 455 | pME-EGFP no stop | EGFP, no stop (to make N-terminal fusions) | 3324 | KMK | 1.2 | |
| 456 | pME-mCherry no stop | mCherry, no stop (to make N-terminal fusions) | 3258 | KMK | 1.2 | |
| 550 | pME-mCherryCAAX | prenylated mCherry | 3321 | KMK/SYC | 1.2 | |
| 3' entry clones, attR2-L3 (kan resistant) | ||||||
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
Notes
- # is the Chien lab stock number
- made by shows who made the construct:
|* CG: Clemens Grabher, Look lab
|* BM: Ben Mangum, Chien lab
|* DSC: Doug Campbell, Chien lab
|* EF: Esther Fujimoto, Chien lab
|* KMK: Kristen Kwan, Chien lab
|* MEH: Melissa Hardy, Chien lab
|* SYC: Seok-yong Choi, Chitnis lab
- ver shows the version of the Tol2kit in which this construct first appeared.
- *:pDestTol2pA and pDestTol2CG have been superseded by pDestTol2pA2 and pDestTol2CG2. They are not formally part of the v1.0 release, and are no longer being distributed.
- **:clone 232 was superseded by clone 450, which lacks the H80D mutation in mCherryCAAX.
| Subsequently, some preps of clone 450 turned out to still be clone 232, so we cleaned it up again, and clone 450 has been superseded by 550.
- MTA? indicates whether an MTA is associated with each clone. "KK", Koichi Kawakami.