PME-H2AmCherry
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Construction Details
pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR; official ZFIN gene name h2afv) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G.
Crude Map
Screenshot from ApE showing locations of components:
234 pME-H2AmCherry
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)