PME-EGFP

Construction Details

pME-EGFP was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

Note that the EGFP sequence is followed by 60 bp of polylinker sequence from pCS2+ before the stop codon. We have confirmed functionally that this cassette produces fluorescent protein.

Crude Map

Screenshot from ApE showing locations of components:

383 pME-EGFP

Download the Sequence

• Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)

Download pME-EGFP