PME-mCherry no stop
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Construction Details
pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
This clone can be used to generate N-terminal mCherry fusions with a gene of interest placed in a 3' clone.
Crude Map
Screenshot from ApE showing locations of components:
456 pME-mCherry no stop
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)