PME-Gal4VP16

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Construction Details

pME-Gal4VP16 was made by PCR amplification of Gal4VP16 from clones made by Reinhard Koester (described in Koester and Fraser, Dev Biol. 2001), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T.

In this construct, the Gal4 DNA binding domain is fused to VP16[413-470], a partially-truncated VP16 transactivation domain. This fusion has reduced toxicity compared to the original Gal4VP16[413-490] from Sadowski et al. 1988. See Ogura et al., Dev Dyn 238:641, 2009 for comparisons of different Gal4VP16 fusions.

Crude Map

Screenshot from ApE showing locations of components:

387 pME-Gal4VP16

Download the Sequence

  • Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)
Download pME-Gal4VP16