P5E-Fse-Asc

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Construction Details

p5E-Fse-Asc was made by PCR of two overlapping primers with att sites and the Fse and Asc recognition sites, followed by a BP reaction. The insert consists of:

GGCCGGCCCTTCGGCGCGCC

that is, an Fse then an Asc site, separated by a 4 bp spacer. Sequencing confirms that all bases of the insert are correct.

Crude Map

Screenshot from ApE showing locations of components:

381 p5E-Fse-Asc.PNG

Download the Sequence

  • Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)
Download p5E-Fse-Asc