Full Clone List
List of Entry and Destination Vectors
Here is a list of the constructs in the Tol2kit. Click on any construct name (second column) to see more information. 12/18/07: Clone pages now include annotated sequence in Genbank format.
Here are insert sequences for entry clones, and 5' and 3' ends for destination clones, along with a description of how to assemble predicted sequences for expression clones.
Constructs in the Tol2kit v1.2 (Nov 2007)
| # | Name | Insert | Size (bp) | Made by | ver | MTA? |
|---|---|---|---|---|---|---|
| 5' entry clones, attL4-R1 (kan resistant) | ||||||
| 299 | p5E-bactin2 | 5.3 kb beta-actin promoter (ubiquitous) | 7950 | KMK | 1.0 | |
| 380 | p5E-h2afx | 1 kb histone2A-X promoter (quasi-ubiquitous) | 3604 | KMK | 1.0 | |
| 382 | p5E-CMV/SP6 | 1 kb CMV/SP6 cassette from pCS2+ | 3704 | KMK | 1.0 | |
| 222 | p5E-hsp70l | 1.5 kb hsp70l promoter for heat-shock induction | 4163 | BM | 1.0 | |
| 327 | p5E-UAS | 10x UAS element and basal promoter for Gal4 response | 3127 | DSC | 1.0 | |
| 228 | p5E-MCS | multiple-cloning site from pBluescript | 2810 | BM | 1.0 | |
| 381 | p5E-Fse-Asc | restriction sites for 8-cutters FseI and AscI | 2663 | EF | 1.0 | |
| Middle Entry Clones, attL1-L2 (kan resistant) | ||||||
| 383 | pME-EGFP | EGFP | 3327 | KMK | 1.0 | |
| 384 | pME-EGFPCAAX | membrane-localized (prenylated) EGFP; fused to 21 aa of H-ras | 3345 | KMK | 1.0 | |
| 385 | pME-nlsEGFP | nuclear-localized EGFP | 3342 | KMK | 1.0 | |
| 386 | pME-mCherry | monomeric red fluorophore mCherry | 3261 | KMK | 1.0 | |
| 232 | pME-mCherryCAAX H80D** | prenylated mCherry (deleterious H80D mutation; superseded by 450) | 3321 | KMK | 1.0 | |
| 233 | pME-nlsmCherry | nuclear-localized mCherry | 3288 | KMK | 1.0 | |
| 234 | pME-H2AmCherry | mCherry fused to zebrafish histone H2A.F/Z | 3651 | KMK | 1.0 | |
| 387 | pME-Gal4VP16 | Gal4 DNA binding domain fused to VP16 transactivation domain | 3204 | EF | 1.0 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 450 | pME-mCherryCAAX** | prenylated mCherry (some preps contam w/ H80D, superseded by #550) | 3321 | KMK/SYC | 1.2 | |
| 455 | pME-EGFP no stop | EGFP, no stop (to make N-terminal fusions) | 3324 | KMK | 1.2 | |
| 456 | pME-mCherry no stop | mCherry, no stop (to make N-terminal fusions) | 3258 | KMK | 1.2 | |
| 550 | pME-mCherryCAAX | prenylated mCherry | 3321 | KMK/SYC | 1.2 | |
| 3' entry clones, attR2-L3 (kan resistant) | ||||||
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
| 237 | pME-MCS | multiple-cloning site from pBluescript | 2765 | BM | 1.1 | |
Notes
- # is the Chien lab stock number
- made by shows who made the construct:
|* CG: Clemens Grabher, Look lab |* BM: Ben Mangum, Chien lab |* DSC: Doug Campbell, Chien lab |* EF: Esther Fujimoto, Chien lab |* KMK: Kristen Kwan, Chien lab |* MEH: Melissa Hardy, Chien lab |* SYC: Seok-yong Choi, Chitnis lab
- ver shows the version of the Tol2kit in which this construct first appeared.
- *:pDestTol2pA and pDestTol2CG have been superseded by pDestTol2pA2 and pDestTol2CG2. They are not formally part of the v1.0 release, and are no longer being distributed.
- **:clone 232 was superseded by clone 450, which lacks the H80D mutation in mCherryCAAX. Subsequently, some preps of clone 450 turned out to still be clone 232, so we cleaned it up again, and clone 450 has been superseded by 550.
- MTA? indicates whether an MTA is associated with each clone. "KK", Koichi Kawakami.