Full Clone List: Difference between revisions

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| '''384'''
| '''384'''
| [[pME-EGFPCAAX|'''pME-EGFPCAAX''']]
| [[pME-EGFPCAAX|'''pME-EGFPCAAX''']]
| 5.3 kb beta-actin promoter (ubiquitous)
| membrane-localized (prenylated) EGFP; fused to 21 aa of H-ras
| 3345
| 3345
| KMK
| KMK
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|
|
|-
|-
| '''384'''
| '''385'''
| [[pME-EGFPCAAX|'''pME-EGFPCAAX''']]
| [[pME-nlsEGFP|'''pME-nlsEGFP''']]
| 5.3 kb beta-actin promoter (ubiquitous)
| nuclear-localized EGFP
| 3345
| 3342
| KMK
| KMK
| 1.0
| 1.0
|
|
|-
|-
| '''384'''
| '''386'''
| [[pME-EGFPCAAX|'''pME-EGFPCAAX''']]
| [[pME-mCherry|'''pME-mCherry''']]
| 5.3 kb beta-actin promoter (ubiquitous)
| monomeric red fluorophore mCherry
| 3345
| 3261
| KMK
| KMK
| 1.0
| 1.0
|
|
|-
|-
| '''384'''
| '''232'''
| [[pME-EGFPCAAX|'''pME-EGFPCAAX''']]
| [[pME-mCherryCAAX H80D**|'''pME-mCherryCAAX H80D**''']]
| 5.3 kb beta-actin promoter (ubiquitous)
| prenylated mCherry (deleterious H80D mutation; superseded by 450)
| 3345
| 3321
| KMK
| KMK
| 1.0
| 1.0
|
|
|-
|-
| '''384'''
| '''233'''
| [[pME-EGFPCAAX|'''pME-EGFPCAAX''']]
| [[pME-nlsmCherry|'''pME-nlsmCherry''']]
| 5.3 kb beta-actin promoter (ubiquitous)
| nuclear-localized mCherry
| 3345
| 3288
| KMK
| KMK
| 1.0
| 1.0
|
|
|-
|-
| '''384'''
| '''234'''
| [[pME-EGFPCAAX|'''pME-EGFPCAAX''']]
| [[pME-H2AmCherry|'''pME-H2AmCherry''']]
| 5.3 kb beta-actin promoter (ubiquitous)
|mCherry fused to zebrafish histone H2A.F/Z
| 3345
| 3651
| KMK
| KMK
| 1.0
| 1.0
|
|
|-
|-
| '''384'''
| '''387'''
| [[pME-EGFPCAAX|'''pME-EGFPCAAX''']]
| [[pME-Gal4VP16|'''pME-Gal4VP16''']]
| 5.3 kb beta-actin promoter (ubiquitous)
| Gal4 DNA binding domain fused to VP16 transactivation domain
| 3345
| 3204
| KMK
| EF
| 1.0
| 1.0
|
|
|-
| '''237'''
| [[pME-MCS|'''pME-MCS''']]
| multiple-cloning site from pBluescript
| 2765
| BM
| 1.1
|
|-
! colspan="7" | '''5' entry clones, attL4-R1 (kan resistant)'''
|-
|-
|}
|}


== Notes ==
== Notes ==

Revision as of 15:40, 6 March 2025

List of Entry and Destination Vectors

Here is a list of the constructs in the Tol2kit. Click on any construct name (second column) to see more information. 12/18/07: Clone pages now include annotated sequence in Genbank format.

Here are insert sequences for entry clones, and 5' and 3' ends for destination clones, along with a description of how to assemble predicted sequences for expression clones.

Constructs in the Tol2kit v1.2 (Nov 2007)

# Name Insert Size (bp) Made by ver MTA?
5' entry clones, attL4-R1 (kan resistant)
299 p5E-bactin2 5.3 kb beta-actin promoter (ubiquitous) 7950 KMK 1.0
380 p5E-h2afx 1 kb histone2A-X promoter (quasi-ubiquitous) 3604 KMK 1.0
382 p5E-CMV/SP6 1 kb CMV/SP6 cassette from pCS2+ 3704 KMK 1.0
222 p5E-hsp70l 1.5 kb hsp70l promoter for heat-shock induction 4163 BM 1.0
327 p5E-UAS 10x UAS element and basal promoter for Gal4 response 3127 DSC 1.0
228 p5E-MCS multiple-cloning site from pBluescript 2810 BM 1.0
381 p5E-Fse-Asc restriction sites for 8-cutters FseI and AscI 2663 EF 1.0
Middle Entry Clones, attL1-L2 (kan resistant)
383 pME-EGFP EGFP 3327 KMK 1.0
384 pME-EGFPCAAX membrane-localized (prenylated) EGFP; fused to 21 aa of H-ras 3345 KMK 1.0
385 pME-nlsEGFP nuclear-localized EGFP 3342 KMK 1.0
386 pME-mCherry monomeric red fluorophore mCherry 3261 KMK 1.0
232 pME-mCherryCAAX H80D** prenylated mCherry (deleterious H80D mutation; superseded by 450) 3321 KMK 1.0
233 pME-nlsmCherry nuclear-localized mCherry 3288 KMK 1.0
234 pME-H2AmCherry mCherry fused to zebrafish histone H2A.F/Z 3651 KMK 1.0
387 pME-Gal4VP16 Gal4 DNA binding domain fused to VP16 transactivation domain 3204 EF 1.0
237 pME-MCS multiple-cloning site from pBluescript 2765 BM 1.1
5' entry clones, attL4-R1 (kan resistant)

Notes

Retrieved from "https://tol2kitkwan.genetics.utah.edu/index.php?title=Full_Clone_List&oldid=70"