Full Clone List
List of Entry and Destination Vectors
Here is a list of the constructs in the Tol2kit. Click on any construct name (second column) to see more information. 12/18/07: Clone pages now include annotated sequence in Genbank format.
Here are insert sequences for entry clones, and 5' and 3' ends for destination clones, along with a description of how to assemble predicted sequences for expression clones.
Constructs in the Tol2kit v1.2 (Nov 2007)
| # | Name | Insert | Size (bp) | Made by | ver | MTA? |
|---|---|---|---|---|---|---|
| 5' entry clones, attL4-R1 (kan resistant) | ||||||
| 299 | p5E-bactin2 | 5.3 kb beta-actin promoter (ubiquitous) | 7950 | KMK | 1.0 | |
| 380 | p5E-h2afx | 1 kb histone2A-X promoter (quasi-ubiquitous) | 3604 | KMK | 1.0 | |
| 382 | p5E-CMV/SP6 | 1 kb CMV/SP6 cassette from pCS2+ | 3704 | KMK | 1.0 | |
| 222 | p5E-hsp70l | 1.5 kb hsp70l promoter for heat-shock induction | 4163 | BM | 1.0 | |
| 327 | p5E-UAS | 10x UAS element and basal promoter for Gal4 response | 3127 | DSC | 1.0 | |
| 228 | p5E-MCS | multiple-cloning site from pBluescript | 2810 | BM | 1.0 | |
| 381 | p5E-Fse-Asc | restriction sites for 8-cutters FseI and AscI | 2663 | EF | 1.0 | |
| Middle Entry Clones, attL1-L2 (kan resistant) | ||||||
| 383 | pME-EGFP | EGFP | 3327 | KMK | 1.0 | |
| 384 | pME-EGFPCAAX | 5.3 kb beta-actin promoter (ubiquitous) | 3345 | KMK | 1.0 | |