Full Clone List: Difference between revisions

From tol2kit for kwan lab
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! colspan="7" | '''Middle Entry Clones, attL1-L2 (kan resistant)'''
! colspan="7" | '''Middle Entry Clones, attL1-L2 (kan resistant)'''
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Revision as of 15:30, 6 March 2025

List of Entry and Destination Vectors

Here is a list of the constructs in the Tol2kit. Click on any construct name (second column) to see more information. 12/18/07: Clone pages now include annotated sequence in Genbank format.

Here are insert sequences for entry clones, and 5' and 3' ends for destination clones, along with a description of how to assemble predicted sequences for expression clones.

Constructs in the Tol2kit v1.2 (Nov 2007)

# Name Insert Size (bp) Made by ver MTA?
5' entry clones, attL4-R1 (kan resistant)
299 p5E-bactin2 5.3 kb beta-actin promoter (ubiquitous) 7950 KMK 1.0
380 p5E-h2afx 1 kb histone2A-X promoter (quasi-ubiquitous) 3604 KMK 1.0
382 p5E-CMV/SP6 1 kb CMV/SP6 cassette from pCS2+ 3704 KMK 1.0
222 p5E-hsp70l 1.5 kb hsp70l promoter for heat-shock induction 4163 BM 1.0
327 p5E-UAS 10x UAS element and basal promoter for Gal4 response 3127 DSC 1.0
228 p5E-MCS multiple-cloning site from pBluescript 2810 BM 1.0
381 p5E-Fse-Asc restriction sites for 8-cutters FseI and AscI 2663 EF 1.0
Middle Entry Clones, attL1-L2 (kan resistant)
383 pME-EGFP EGFP 3327 KMK 1.0
384 pME-EGFPCAAX 5.3 kb beta-actin promoter (ubiquitous) 3345 KMK 1.0
384 pME-EGFPCAAX 5.3 kb beta-actin promoter (ubiquitous) 3345 KMK 1.0
384 pME-EGFPCAAX 5.3 kb beta-actin promoter (ubiquitous) 3345 KMK 1.0
384 pME-EGFPCAAX 5.3 kb beta-actin promoter (ubiquitous) 3345 KMK 1.0
384 pME-EGFPCAAX 5.3 kb beta-actin promoter (ubiquitous) 3345 KMK 1.0
384 pME-EGFPCAAX 5.3 kb beta-actin promoter (ubiquitous) 3345 KMK 1.0
384 pME-EGFPCAAX 5.3 kb beta-actin promoter (ubiquitous) 3345 KMK 1.0

Notes