P3E-IRES-EGFPCAAXpA
Construction Details
p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing finds a single bp change in the IRES (G481A), but this construct is functional.
Crude Map
Screenshot from Sequencher showing locations of components: P3E-IRES-EGFPCAAXpA.png
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)