P3E-IRES-EGFPCAAXpA
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Construction Details
p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing finds a single bp change in the IRES (G481A), but this construct is functional.
Crude Map
Screenshot from ApE showing locations of components:
390 p3E-IRES-EGFPCAAXpA
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)