PME-H2AmCherry
Construction Details
pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR; official ZFIN gene name h2afv) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G.
Crude Map
Screenshot from Sequencher showing locations of components: PME-H2AmCherry.png
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)