Full Clone List: Difference between revisions
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|- | |- | ||
| '''382''' | | '''382''' | ||
| [[p5E- | | [[p5E-CMV/SP6|'''p5E-CMV/SP6''']] | ||
| | | 1 kb CMV/SP6 cassette from pCS2+ | ||
| | | 3704 | ||
| KMK | | KMK | ||
| 1.0 | | 1.0 | ||
|- | |- | ||
| '''222''' | | '''222''' | ||
| [[p5E- | | [[p5E-hsp70l|'''p5E'''-''hsp70l'']] | ||
| 5 | | 1.5 kb hsp70l promoter for heat-shock induction | ||
| | | 4163 | ||
| BM | | BM | ||
| 1.0 | | 1.0 | ||
|- | |- | ||
| '''327''' | | '''327''' | ||
| [[p5E- | | [[p5E-UAS|'''p5E-UAS''']] | ||
| | | 10x UAS element and basal promoter for Gal4 response | ||
| | | 3127 | ||
| DSC | | DSC | ||
| 1.0 | | 1.0 | ||
Revision as of 18:44, 5 March 2025
List of Entry and Destination Vectors
Here is a list of the constructs in the Tol2kit. Click on any construct name (second column) to see more information. 12/18/07: Clone pages now include annotated sequence in Genbank format.
Here are insert sequences for entry clones, and 5' and 3' ends for destination clones, along with a description of how to assemble predicted sequences for expression clones.
Constructs in the Tol2kit v1.2 (Nov 2007)
| # | Name | Insert | Size (bp) | Made by | ver | MTA? |
|---|---|---|---|---|---|---|
| 5' entry clones, attL4-R1 (kan resistant) | ||||||
| 299 | p5E-bactin2 | 5.3 kb beta-actin promoter (ubiquitous) | 7950 | KMK | 1.0 | |
| 380 | p5E-h2afx | 1 kb histone2A-X promoter (quasi-ubiquitous) | 3604 | KMK | 1.0 | |
| 382 | p5E-CMV/SP6 | 1 kb CMV/SP6 cassette from pCS2+ | 3704 | KMK | 1.0 | |
| 222 | p5E-hsp70l | 1.5 kb hsp70l promoter for heat-shock induction | 4163 | BM | 1.0 | |
| 327 | p5E-UAS | 10x UAS element and basal promoter for Gal4 response | 3127 | DSC | 1.0 | |
| 228 | p5E-bactin2 | 5.3 kb beta-actin promoter (ubiquitous) | 7950 | BM | 1.0 | |
| 381 | p5E-bactin2 | 5.3 kb beta-actin promoter (ubiquitous) | 7950 | EF | 1.0 | |
| Middle Entry Clones, attL1-L2 (kan resistant) | ||||||
| 299 | p5E-bactin2 | 5.3 kb beta-actin promoter (ubiquitous) | 7950 | KMK | 1.0 | |
| 299 | p5E-bactin2 | 5.3 kb beta-actin promoter (ubiquitous) | 7950 | KMK | 1.0 | |