PME-H2AmCherry: Difference between revisions
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== Crude Map == | == Crude Map == | ||
Screenshot from | Screenshot from ApE showing locations of components: | ||
:[[File:234 pME-H2AmCherrycrop.PNG|thumb|none|350px|234 pME-H2AmCherry]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:234.pME-H2AmCherry.zip|Download pME-H2AmCherry]] | : [[Media:234.pME-H2AmCherry.zip|Download pME-H2AmCherry]] | ||
Latest revision as of 20:35, 29 May 2025
Construction Details
pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR; official ZFIN gene name h2afv) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G.
Crude Map
Screenshot from ApE showing locations of components:
234 pME-H2AmCherry
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)