P3E-IRES-EGFPCAAXpA: Difference between revisions
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== Crude Map == | == Crude Map == | ||
Screenshot from | Screenshot from ApE showing locations of components: | ||
:[[File:390 p3E-IRES-EGFPCAAXpA.PNG|thumb|none|350px|390 p3E-IRES-EGFPCAAXpA]] | :[[File:390 p3E-IRES-EGFPCAAXpA.PNG|thumb|none|350px|390 p3E-IRES-EGFPCAAXpA]] | ||
Latest revision as of 20:02, 29 May 2025
Construction Details
p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing finds a single bp change in the IRES (G481A), but this construct is functional.
Crude Map
Screenshot from ApE showing locations of components:
390 p3E-IRES-EGFPCAAXpA
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)