PME-EGFP: Difference between revisions
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Screenshot from ApE showing locations of components: | Screenshot from ApE showing locations of components: | ||
:[[File:383 pME-EGFP.PNG|thumb|none| | :[[File:383 pME-EGFP.PNG|thumb|none|400px|383 pME-EGFP]] | ||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:383.pME-EGFP.zip|Download pME-EGFP]] | : [[Media:383.pME-EGFP.zip|Download pME-EGFP]] | ||
Revision as of 20:11, 29 May 2025
Construction Details
pME-EGFP was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
- Note that the EGFP sequence is followed by 60 bp of polylinker sequence from pCS2+ before the stop codon. We have confirmed functionally that this cassette produces fluorescent protein.
Crude Map
Screenshot from ApE showing locations of components:
383 pME-EGFP
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)