PME-H2AmCherry: Difference between revisions
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Hggenusrwki (talk | contribs) Created page with "== Construction Details == This template is used to display DNA sequence information in a standardized format. == Crude Map == photo showing locations of components == Sequence == * Annotated Sequence: (Genbank format) link * Full-Length Sequence with individual component sequences included: (FASTA file) link == Reference == This template is used to display DNA sequence information in a standardized format." |
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== Construction Details == | == Construction Details == | ||
pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR; official ZFIN gene name h2afv) to the N terminus of | |||
mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G. | |||
== Crude Map == | == Crude Map == | ||
Screenshot from ApE showing locations of components: | |||
:[[File:234 pME-H2AmCherrycrop.PNG|thumb|none|350px|234 pME-H2AmCherry]] | |||
* Full-Length Sequence with individual component sequences included | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | |||
: [[Media:234.pME-H2AmCherry.zip|Download pME-H2AmCherry]] | |||
Latest revision as of 20:35, 29 May 2025
Construction Details
pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR; official ZFIN gene name h2afv) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G.
Crude Map
Screenshot from ApE showing locations of components:
234 pME-H2AmCherry
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)