PME-Gal4VP16: Difference between revisions
Jump to navigation
Jump to search
Hggenusrwki (talk | contribs) No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
== Crude Map == | == Crude Map == | ||
Screenshot from | Screenshot from ApE showing locations of components: | ||
:[[File:387 pME-Gal4VP16crop.PNG|thumb|none|350px|387 pME-Gal4VP16]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:387.pME-Gal4VP16.zip|Download pME-Gal4VP16]] | : [[Media:387.pME-Gal4VP16.zip|Download pME-Gal4VP16]] | ||
Latest revision as of 20:37, 29 May 2025
Construction Details
pME-Gal4VP16 was made by PCR amplification of Gal4VP16 from clones made by Reinhard Koester (described in Koester and Fraser, Dev Biol. 2001), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T.
In this construct, the Gal4 DNA binding domain is fused to VP16[413-470], a partially-truncated VP16 transactivation domain. This fusion has reduced toxicity compared to the original Gal4VP16[413-490] from Sadowski et al. 1988. See Ogura et al., Dev Dyn 238:641, 2009 for comparisons of different Gal4VP16 fusions.
Crude Map
Screenshot from ApE showing locations of components:
387 pME-Gal4VP16
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)