P3E-IRES-EGFPCAAXpA: Difference between revisions
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Hggenusrwki (talk | contribs) Created page with "== Construction Details == This template is used to display DNA sequence information in a standardized format. == Crude Map == photo showing locations of components == Sequence == * Annotated Sequence: (Genbank format) link * Full-Length Sequence with individual component sequences included: (FASTA file) link == Reference == This template is used to display DNA sequence information in a standardized format." |
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== Construction Details == | == Construction Details == | ||
p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing finds a single bp change in the IRES (G481A), but this construct is functional. | |||
== Crude Map == | == Crude Map == | ||
Screenshot from ApE showing locations of components: | |||
:[[File:390 p3E-IRES-EGFPCAAXpA.PNG|thumb|none|350px|390 p3E-IRES-EGFPCAAXpA]] | |||
* Full-Length Sequence with individual component sequences included | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | |||
: [[Media:390.p3E-IRES-EGFPCAAXpA.zip|Download p3E-IRES-EGFPCAAXpA]] | |||
Latest revision as of 20:02, 29 May 2025
Construction Details
p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing finds a single bp change in the IRES (G481A), but this construct is functional.
Crude Map
Screenshot from ApE showing locations of components:
390 p3E-IRES-EGFPCAAXpA
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)