P3E-mCherrypA: Difference between revisions
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== Crude Map == | == Crude Map == | ||
Screenshot from | Screenshot from ApE showing locations of components: | ||
:[[File:388 p3E-mCherrypA.PNG|thumb|none|350px|388 p3E-mCherrypA.PNG]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:388.p3E-mCherrypA.zip|Download p3E-mCherrypA]] | : [[Media:388.p3E-mCherrypA.zip|Download p3E-mCherrypA]] | ||
Latest revision as of 20:02, 29 May 2025
Construction Details
p3E-mCherrypA was made by PCR amplification of mCherry and an SV40 late polyadenylation signal from a pCS2+-based construct, using primers to add att sites, followed by a BP reaction. Sequencing finds a single base PCR error, encoding an E7D mutation in mCherry. Functional tests of Cterminal fusion proteins made with this construct show that they yield fluorescent protein.
The mCherry sequence includes a start codon but not a good Kozak consensus (unlike p3E-EGFP-pA, which has both).
Crude Map
Screenshot from ApE showing locations of components:
388 p3E-mCherrypA.PNG
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)