P3E-mCherrypA
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Construction Details
p3E-mCherrypA was made by PCR amplification of mCherry and an SV40 late polyadenylation signal from a pCS2+-based construct, using primers to add att sites, followed by a BP reaction. Sequencing finds a single base PCR error, encoding an E7D mutation in mCherry. Functional tests of Cterminal fusion proteins made with this construct show that they yield fluorescent protein.
The mCherry sequence includes a start codon but not a good Kozak consensus (unlike p3E-EGFP-pA, which has both).
Crude Map
Screenshot from ApE showing locations of components:
388 p3E-mCherrypA.PNG
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)