View source for PME-EGFP

== Construction Details ==
pME-EGFP was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

: Note that the EGFP sequence is followed by 60 bp of polylinker sequence from pCS2+ before the stop codon. We have confirmed functionally that this cassette produces fluorescent protein.

== Crude Map ==
Screenshot from ApE showing locations of components:

:[[File:383 pME-EGFP.PNG|thumb|none|400px|383 pME-EGFP]]

==Download the Sequence ==
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file)
: [[Media:383.pME-EGFP.zip|Download pME-EGFP]]