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Combined display of all available logs of tol2kit for kwan lab. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).

• 16:48, 5 March 2025 Hggenusrwki talk contribs created page Protocols (Created page with "== Growing up Clones == Entry clones are kanamycin-resistant and can be transformed and grown in any standard E. coli strain. For a single lab's use, minipreps (e.g. using Qiagen miniprep columns) are sufficient for the entry vectors; you will use very little DNA for each LR reaction. If you run low, you can always just miniprep again. However, note that destination vectors have an ampicillin resistance gene in the backbone, donor vectors have a kanamycin resistance gene...")