P3E-polyA

From tol2kit for kwan lab
Revision as of 20:01, 29 May 2025 by KmKwan (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Construction Details

p3E-polyA was made by PCR amplification of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

Crude Map

Screenshot from ApE showing locations of components:

302 p3E-polyA

Download the Sequence

  • Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)
Download p3E-polyA