P5E-h2afx
Construction Details
p5E-h2afx was made by PCR amplification of 1 kb genomic DNA immediately upstream of the start codon of h2afx (NM_201073), using primers that added att sites, followed by a BP reaction. This genomic element was originally isolated by John Parant in Joe Yost's lab. Sequencing confirms that the insert is correct, although there are numerous single-base changes compared to the Sanger genomic sequence (presumably representing SNPs).
Expression data for this gene from the Thisses (see ZFIN (http://zfin.org/cgi-bin/webdriver?MIval=aaxpatselect.apg&query_results=exist&START=0&searchtype=contains&gene_name=h2afx)) that shows that h2afx is expressed throughout the embryo in presumptive proliferative regions.
Crude Map
Screenshot from Sequencher showing locations of components: P5E-h2afx.png
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)
Reference
Higashijima S, Okamoto H, Ueno N, Hotta Y, Eguchi G. (1997) Dev Biol.15;192:289-99. "High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin."