P5E-bactin2
Construction Details
p5E-MCS was made by PCR of the pBSII SK+ multiple cloning site, from T7 to T3 primers, followed by a BP reaction with pDONR P4-P1R. Sequencing confirms that all bases of the insert are correct.
M13F, M13R, and T3 should all work as sequencing primers.
Unfortunately, the backbone of P4-P1R contains recognition sites for several potentially useful restriction enzymes. The restriction sites from the Bluescript MCS that remain unique in p5E-MCS are:
Asp718-KpnI-DraII-XhoI-SalI-Bsp106-HindIII-SmaI-BamHI-SpeI-SacII-BstXI
Crude Map
Screenshot from Sequencher showing locations of components: P5E-MCS.png
Download the Sequence
- Annotated Sequence (Genbank format) and Full-Length Sequence with individual component sequences included (FASTA file)
Reference
This template is used to display DNA sequence information in a standardized format.