PME-nlsmCherry

Construction Details

pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction.

Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence).

Crude Map

photo showing locations of components PME-nlsmCherry.png

Sequence

• Annotated Sequence: (Genbank format)

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• Full-Length Sequence with individual component sequences included: (FASTA file)

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Reference

This template is used to display DNA sequence information in a standardized format.