PME-EGFP no stop: Difference between revisions
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== Crude Map == | == Crude Map == | ||
Screenshot from | Screenshot from ApE showing locations of components: | ||
:[[File:455 pME-EGFPnostopcrop.PNG|thumb|none|350px|455 pME-EGFP no stop]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:455.pME-EGFP no stop.zip|Download pME-EGFP no stop]] | : [[Media:455.pME-EGFP no stop.zip|Download pME-EGFP no stop]] | ||
Latest revision as of 20:42, 29 May 2025
Construction Details
pME-EGFP no stop was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
This clone can be used to generate N-terminal EGFP fusions with a gene of interest placed in a 3' clone.
Crude Map
Screenshot from ApE showing locations of components:
455 pME-EGFP no stop
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)