PME-mCherryCAAX**: Difference between revisions
Hggenusrwki (talk | contribs) No edit summary |
No edit summary |
||
| Line 6: | Line 6: | ||
Oct 2009: We reisolated the correct clone, now named clone 550, and have been distributing this for quite a while. Clone 450 is now obsolete. | Oct 2009: We reisolated the correct clone, now named clone 550, and have been distributing this for quite a while. Clone 450 is now obsolete. | ||
'''This clone was replaced by 550, which lacks this mutation.''' | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:550.pME-mCherryCAAX.zip|Download pME-mCherryCAAX]] | : [[Media:550.pME-mCherryCAAX.zip|Download pME-mCherryCAAX]] | ||
Latest revision as of 20:30, 29 May 2025
Construction Details
pME-mCherryCAAX was made by PCR amplification of mCherry-CAAX, using primers to add att sites, followed by a BP reaction. mCherry- CAAX is mCherry with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. The original maxiprep that was distributed (clone 232) had an H80D point mutation. Nov 2007: The new version (clone 450) has had this mutation fixed and has no remaining mutations (thanks a lot to Seok-yong Choi).
Aug 2008: To our dismay, we realized that our current maxiprep of clone 450 again has mutation C238G, encoding the H80D amino acid change. We are currently reisolating the correct clone.
Oct 2009: We reisolated the correct clone, now named clone 550, and have been distributing this for quite a while. Clone 450 is now obsolete.
This clone was replaced by 550, which lacks this mutation.
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)