P3E-IRES-nlsEGFPpA: Difference between revisions
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== Crude Map == | == Crude Map == | ||
Screenshot from Sequencher showing locations of components: | Screenshot from Sequencher showing locations of components: | ||
:[[File:391 p3E-IRES-nlsEGFPpA.PNG|thumb|none|350px|391 p3E-IRES-nlsEGFPpA.PNG]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:391.p3E-IRES-nlsEGFPpA.zip|Download p3E-IRES-nlsEGFPpA]] | : [[Media:391.p3E-IRES-nlsEGFPpA.zip|Download p3E-IRES-nlsEGFPpA]] | ||
Revision as of 18:32, 29 May 2025
Construction Details
p3E-IRES-nlsEGFPpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and nlsEGFP-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the coding sequence are correct. There is a single base change in the IRES sequence (G481A), but we have confirmed functionally that this cassette produces fluorescent, nuclear-localized protein.
Crude Map
Screenshot from Sequencher showing locations of components:
391 p3E-IRES-nlsEGFPpA.PNG
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)