P3E-polyA: Difference between revisions

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== Crude Map ==
== Crude Map ==
Screenshot from Sequencher showing locations of components:
Screenshot from Sequencher showing locations of components:
P3E-polyA.png
 
:[[File:302 p3E-polyA.PNG|thumb|none|350px|302 p3E-polyA]]


==Download the Sequence ==
==Download the Sequence ==
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file)
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file)
: [[Media:302.p3E-polyA.zip|Download p3E-polyA]]
: [[Media:302.p3E-polyA.zip|Download p3E-polyA]]

Revision as of 18:14, 29 May 2025

Construction Details

p3E-polyA was made by PCR amplification of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

Crude Map

Screenshot from Sequencher showing locations of components:

302 p3E-polyA

Download the Sequence

  • Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)
Download p3E-polyA