PCS2FA-transposase: Difference between revisions
Jump to navigation
Jump to search
Hggenusrwki (talk | contribs) No edit summary Tag: Reverted |
Hggenusrwki (talk | contribs) No edit summary Tag: Manual revert |
||
| Line 7: | Line 7: | ||
Screenshot from Sequencher showing locations of components: | Screenshot from Sequencher showing locations of components: | ||
PCS2FA-transposase.png | PCS2FA-transposase.png | ||
:[[File:396 pCS2FA-Tol2transposase.PNG|thumb|none| | :[[File:396 pCS2FA-Tol2transposase.PNG|thumb|none|250px|396 pCS2FA-Tol2transposase]] | ||
[{{fullurl:File:396 pCS2FA-Tol2transposase.PNG|direct}} [[File:396 pCS2FA-Tol2transposase.PNG|thumb|left|250px|Caption]]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:396.pCS2FA-transposase.zip|Download pCS2FA-transposase]] | : [[Media:396.pCS2FA-transposase.zip|Download pCS2FA-transposase]] | ||
Revision as of 17:46, 29 May 2025
Construction Details
pCS2FA-transposase was made by PCRing the Tol2 transpoase cds from the original Kawakami clone, adding restriction sites, then conventionally subcloned into pCS2FA, a version of pCS2+ modified by adding FseI and AscI sites. Sequencing confirms that all bases of the insert are correct.
The original transposase clone included start and stop codons upstream of the transposase cds, and a polyA sequence (AAAAA) rather than a polyadenylation signal.
Crude Map
Screenshot from Sequencher showing locations of components: PCS2FA-transposase.png
396 pCS2FA-Tol2transposase
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)