PCS2FA-transposase: Difference between revisions
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Hggenusrwki (talk | contribs) Created page with "== Construction Details == This template is used to display DNA sequence information in a standardized format. == Crude Map == photo showing locations of components == Sequence == * Annotated Sequence: (Genbank format) link * Full-Length Sequence with individual component sequences included: (FASTA file) link == Reference == This template is used to display DNA sequence information in a standardized format." |
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== Construction Details == | == Construction Details == | ||
pCS2FA-transposase was made by PCRing the Tol2 transpoase cds from the original Kawakami clone, adding restriction sites, then conventionally subcloned into pCS2FA, a version of pCS2+ modified by adding FseI and AscI sites. Sequencing confirms that all bases of the insert are correct. | |||
The original transposase clone included start and stop codons upstream of the transposase cds, and a polyA sequence (AAAAA) rather than a polyadenylation ''signal''. | |||
== Crude Map == | == Crude Map == | ||
Screenshot from Sequencher showing locations of components: | |||
PCS2FA-transposase.png | |||
== | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | |||
: [[Media:233._pME-nlsmCherry.zip|Download pCS2FA-transposase]] | |||
Revision as of 16:46, 11 March 2025
Construction Details
pCS2FA-transposase was made by PCRing the Tol2 transpoase cds from the original Kawakami clone, adding restriction sites, then conventionally subcloned into pCS2FA, a version of pCS2+ modified by adding FseI and AscI sites. Sequencing confirms that all bases of the insert are correct.
The original transposase clone included start and stop codons upstream of the transposase cds, and a polyA sequence (AAAAA) rather than a polyadenylation signal.
Crude Map
Screenshot from Sequencher showing locations of components: PCS2FA-transposase.png
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)