P5E-h2afx: Difference between revisions
Hggenusrwki (talk | contribs) Created page with "== Construction Details == This template is used to display DNA sequence information in a standardized format. == Crude Map == photo showing locations of components == Sequence == * Annotated Sequence: (Genbank format) link * Full-Length Sequence with individual component sequences included: (FASTA file) link == Reference == This template is used to display DNA sequence information in a standardized format." |
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== Construction Details == | == Construction Details == | ||
This | p5E-''h2afx'' was made by PCR amplification of 1 kb genomic DNA immediately upstream of the start codon of h2afx (NM_201073), using primers that added att sites, followed by a BP reaction. This genomic element was originally isolated by John Parant in Joe Yost's lab. Sequencing confirms that the insert is correct, although there are numerous single-base changes compared to the Sanger genomic sequence (presumably representing SNPs). | ||
Expression data for this gene from the Thisses (see ZFIN (http://zfin.org/cgi-bin/webdriver?MIval=aaxpatselect.apg&query_results=exist&START=0&searchtype=contains&gene_name=h2afx)) that shows that h2afx is expressed throughout the embryo in presumptive proliferative regions. | |||
== Crude Map == | == Crude Map == | ||
Screenshot from Sequencher showing locations of components: | |||
P5E-h2afx.png | |||
== Sequence == | ==Download the Sequence == | ||
* Annotated Sequence | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:233._pME-nlsmCherry.zip|Download p5E-''h2afx'']] | |||
== Reference == | == Reference == | ||
Higashijima S, Okamoto H, Ueno N, Hotta Y, Eguchi G. (1997) Dev Biol.15;192:289-99. "High-frequency generation of transgenic zebrafish which | |||
reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin." | |||
Revision as of 16:09, 11 March 2025
Construction Details
p5E-h2afx was made by PCR amplification of 1 kb genomic DNA immediately upstream of the start codon of h2afx (NM_201073), using primers that added att sites, followed by a BP reaction. This genomic element was originally isolated by John Parant in Joe Yost's lab. Sequencing confirms that the insert is correct, although there are numerous single-base changes compared to the Sanger genomic sequence (presumably representing SNPs).
Expression data for this gene from the Thisses (see ZFIN (http://zfin.org/cgi-bin/webdriver?MIval=aaxpatselect.apg&query_results=exist&START=0&searchtype=contains&gene_name=h2afx)) that shows that h2afx is expressed throughout the embryo in presumptive proliferative regions.
Crude Map
Screenshot from Sequencher showing locations of components: P5E-h2afx.png
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)
Reference
Higashijima S, Okamoto H, Ueno N, Hotta Y, Eguchi G. (1997) Dev Biol.15;192:289-99. "High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin."