P5E-bactin2: Difference between revisions
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== Construction Details == | == Construction Details == | ||
''bactin2'' genomic sequence was derived from a 5.3 kb clone (gift of Ken Poss), which was derived in turn from a 10 kb upstream construct from Shinichi Higashijima. | |||
p5E-bactin2 was made by PCR of the bactin2 upstream genomic sequence (using primers to add att sites), followed by a BP reaction. Upstream sequence includes 3.8 kb upstream of transcriptional start, a small 5' UTR exon, a 1.4 kb intron, and then 38 bp of 5' UTR, terminating immediately before the native start codon. Sequencing confirms that the ends of the insert are correct. | |||
== Crude Map == | == Crude Map == | ||
Screenshot from Sequencher showing locations of components: | Screenshot from Sequencher showing locations of components: | ||
P5E- | P5E-bactin2.png | ||
==Download the Sequence == | ==Download the Sequence == | ||
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== Reference == | == Reference == | ||
Higashijima S, Okamoto H, Ueno N, Hotta Y, Eguchi G. (1997) Dev Biol.15;192:289-99. "High-frequency generation of transgenic zebrafish which | |||
reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin." | |||
Revision as of 16:05, 11 March 2025
Construction Details
bactin2 genomic sequence was derived from a 5.3 kb clone (gift of Ken Poss), which was derived in turn from a 10 kb upstream construct from Shinichi Higashijima.
p5E-bactin2 was made by PCR of the bactin2 upstream genomic sequence (using primers to add att sites), followed by a BP reaction. Upstream sequence includes 3.8 kb upstream of transcriptional start, a small 5' UTR exon, a 1.4 kb intron, and then 38 bp of 5' UTR, terminating immediately before the native start codon. Sequencing confirms that the ends of the insert are correct.
Crude Map
Screenshot from Sequencher showing locations of components: P5E-bactin2.png
Download the Sequence
- Annotated Sequence (Genbank format) and Full-Length Sequence with individual component sequences included (FASTA file)
Reference
Higashijima S, Okamoto H, Ueno N, Hotta Y, Eguchi G. (1997) Dev Biol.15;192:289-99. "High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin."