P3E-IRES-EGFPpA: Difference between revisions
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Hggenusrwki (talk | contribs) Created page with "== Construction Details == This template is used to display DNA sequence information in a standardized format. == Crude Map == photo showing locations of components == Sequence == * Annotated Sequence: (Genbank format) link * Full-Length Sequence with individual component sequences included: (FASTA file) link == Reference == This template is used to display DNA sequence information in a standardized format." |
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== Construction Details == | == Construction Details == | ||
p3E-IRES-EGFPpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and EGFP-SV40 late polyA, followed by a BP reaction. Note that the EGFP sequence is followed by 60 bp of polylinker sequence from pCS2+ before the stop codon. We have confirmed functionally that this cassette produces fluorescent protein. Sequencing confirms that all bases of the insert are correct. | |||
== Crude Map == | == Crude Map == | ||
Screenshot from Sequencher showing locations of components: | |||
P3E-IRES-EGFPpA.png | |||
== Sequence == | ==Download the Sequence == | ||
* Annotated Sequence | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:389.p3E-IRES-EGFPpA.zip|Download p3E-IRES-EGFPpA]] | |||
Revision as of 16:34, 13 March 2025
Construction Details
p3E-IRES-EGFPpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and EGFP-SV40 late polyA, followed by a BP reaction. Note that the EGFP sequence is followed by 60 bp of polylinker sequence from pCS2+ before the stop codon. We have confirmed functionally that this cassette produces fluorescent protein. Sequencing confirms that all bases of the insert are correct.
Crude Map
Screenshot from Sequencher showing locations of components: P3E-IRES-EGFPpA.png
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)