PME-mCherryCAAX: Difference between revisions
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== Construction Details == | == Construction Details == | ||
pME-mCherryCAAX was made by PCR amplification of mCherry-CAAX, using primers to add att sites, followed by a BP reaction. mCherry-CAAX is mCherry with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. The original maxiprep that was distributed (clone 232) had an H80D point mutation. Nov 2007: The new version (clone 450) has had this mutation fixed and has no remaining mutations (thanks a lot to Seok-yong Choi). | |||
Aug 2008: To our dismay, we realized that our current maxiprep of clone 450 again has mutation C238G, encoding the H80D amino acid change. We are currently reisolating the correct clone. | |||
Oct 2009: We reisolated the correct clone, now named clone 550, and have been distributing this for quite a while. Clone 450 is now obsolete. | |||
== Crude Map == | == Crude Map == | ||
Screenshot from Sequencher showing locations of components: | Screenshot from Sequencher showing locations of components: | ||
PME-mCherryCAAX.png | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:299.p5E-bactin2.zip|Download | : [[Media:299.p5E-bactin2.zip|Download ''550'' pME-mCherryCAAX]] | ||
Revision as of 14:44, 17 March 2025
Construction Details
pME-mCherryCAAX was made by PCR amplification of mCherry-CAAX, using primers to add att sites, followed by a BP reaction. mCherry-CAAX is mCherry with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. The original maxiprep that was distributed (clone 232) had an H80D point mutation. Nov 2007: The new version (clone 450) has had this mutation fixed and has no remaining mutations (thanks a lot to Seok-yong Choi).
Aug 2008: To our dismay, we realized that our current maxiprep of clone 450 again has mutation C238G, encoding the H80D amino acid change. We are currently reisolating the correct clone.
Oct 2009: We reisolated the correct clone, now named clone 550, and have been distributing this for quite a while. Clone 450 is now obsolete.
Crude Map
Screenshot from Sequencher showing locations of components: PME-mCherryCAAX.png
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)