P3E-IRES-EGFPpA: Difference between revisions
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== Crude Map == | == Crude Map == | ||
Screenshot from Sequencher showing locations of components: | Screenshot from Sequencher showing locations of components: | ||
:[[File:389 p3E-IRES-EGFPpA.PNG|thumb|none|350px|389 p3E-IRES-EGFPpA]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:389.p3E-IRES-EGFPpA.zip|Download p3E-IRES-EGFPpA]] | : [[Media:389.p3E-IRES-EGFPpA.zip|Download p3E-IRES-EGFPpA]] | ||
Revision as of 18:27, 29 May 2025
Construction Details
p3E-IRES-EGFPpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and EGFP-SV40 late polyA, followed by a BP reaction. Note that the EGFP sequence is followed by 60 bp of polylinker sequence from pCS2+ before the stop codon. We have confirmed functionally that this cassette produces fluorescent protein. Sequencing confirms that all bases of the insert are correct.
Crude Map
Screenshot from Sequencher showing locations of components:
389 p3E-IRES-EGFPpA
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)