P3E-MTpA: Difference between revisions
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== Crude Map == | == Crude Map == | ||
Screenshot from Sequencher showing locations of components: | Screenshot from Sequencher showing locations of components: | ||
:[[File:229 p3E-MTpA.PNG|thumb|none|350px|229 p3E-MTpA]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:229.p3E-MTpA.zip|Download p3E-MTpA]] | : [[Media:229.p3E-MTpA.zip|Download p3E-MTpA]] | ||
Revision as of 18:16, 29 May 2025
Construction Details
p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 late polyadenylation signal from pCS2+MT, using primers to add att sequences, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Crude Map
Screenshot from Sequencher showing locations of components:
229 p3E-MTpA
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)