PME-nlsmCherry: Difference between revisions
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PME-nlsmCherry.png | PME-nlsmCherry.png | ||
== Sequence == | ==Download the Sequence == | ||
* Annotated Sequence: (Genbank format) | * Annotated Sequence: (Genbank format) and Full-Length Sequence with individual component sequences included: (FASTA file) | ||
[[Media:233._pME-nlsmCherry.zip|Download pME-nlsmCherry.gb]] | : [[Media:233._pME-nlsmCherry.zip|Download pME-nlsmCherry.gb]] | ||
== Reference == | == Reference == | ||
This template is used to display DNA sequence information in a standardized format. | This template is used to display DNA sequence information in a standardized format. | ||
Revision as of 15:39, 11 March 2025
Construction Details
pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction.
Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence).
Crude Map
photo showing locations of components PME-nlsmCherry.png
Download the Sequence
- Annotated Sequence: (Genbank format) and Full-Length Sequence with individual component sequences included: (FASTA file)
Reference
This template is used to display DNA sequence information in a standardized format.