P5E-CMV/SP6: Difference between revisions
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Hggenusrwki (talk | contribs) Created page with "== Construction Details == This template is used to display DNA sequence information in a standardized format. == Crude Map == photo showing locations of components == Sequence == * Annotated Sequence: (Genbank format) link * Full-Length Sequence with individual component sequences included: (FASTA file) link == Reference == This template is used to display DNA sequence information in a standardized format." |
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== Construction Details == | == Construction Details == | ||
P5E-CMV/SP6 was made by PCR amplification of the sCMV and SP6 promoters from pCS2+, using primers that added att sites, followed by a BP reaction. | |||
This 5' element can be used to build pCS2-like constructs, either to make capped mRNA for injection, or to drive expression from the CMV promoter after DNA injection. | |||
The insert has been completely sequenced, revealing two point mutations (T889C and G1508T) in the CMV promoter; however, DNA injection of constructs made with this clone show that the CMV appears to be functional. | |||
== Crude Map == | |||
Screenshot from ApE showing locations of components: | |||
:[[File:382 p5E-CMV-SP6.PNG|thumb|none|350px|382 p5E-CMV-SP6]] | |||
== | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | |||
: [[Media:382.p5E-CMV-SP6.zip|Download p5E-CMV-SP6]] | |||
Latest revision as of 19:54, 29 May 2025
Construction Details
P5E-CMV/SP6 was made by PCR amplification of the sCMV and SP6 promoters from pCS2+, using primers that added att sites, followed by a BP reaction.
This 5' element can be used to build pCS2-like constructs, either to make capped mRNA for injection, or to drive expression from the CMV promoter after DNA injection.
The insert has been completely sequenced, revealing two point mutations (T889C and G1508T) in the CMV promoter; however, DNA injection of constructs made with this clone show that the CMV appears to be functional.
Crude Map
Screenshot from ApE showing locations of components:
382 p5E-CMV-SP6
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)