P5E-bactin2: Difference between revisions
Hggenusrwki (talk | contribs) Created page with "== Construction Details == This template is used to display DNA sequence information in a standardized format. == Crude Map == photo showing locations of components == Sequence == * Annotated Sequence: (Genbank format) link * Full-Length Sequence with individual component sequences included: (FASTA file) link == Reference == This template is used to display DNA sequence information in a standardized format." |
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== Construction Details == | == Construction Details == | ||
''bactin2'' genomic sequence was derived from a 5.3 kb clone (gift of Ken Poss), which was derived in turn from a 10 kb upstream construct from Shinichi Higashijima. | |||
p5E-bactin2 was made by PCR of the bactin2 upstream genomic sequence (using primers to add att sites), followed by a BP reaction. Upstream sequence includes 3.8 kb upstream of transcriptional start, a small 5' UTR exon, a 1.4 kb intron, and then 38 bp of 5' UTR, terminating immediately before the native start codon. Sequencing confirms that the ends of the insert are correct. | |||
== Crude Map == | == Crude Map == | ||
Screenshot from ApE showing locations of components: | |||
:[[File:299 p5E-bactin2.PNG|thumb|none|350px|299 p5E-bactin2]] | |||
* Full-Length Sequence with individual component sequences included | ==Download the Sequence == | ||
* '''Annotated Sequence''' (Genbank format) and '''Full-Length Sequence with individual component sequences included''' (FASTA file) | |||
: [[Media:299.p5E-bactin2.zip|Download p5E-''bactin2'']] | |||
== Reference == | == Reference == | ||
Higashijima S, Okamoto H, Ueno N, Hotta Y, Eguchi G. (1997) Dev Biol.15;192:289-99. "High-frequency generation of transgenic zebrafish which | |||
reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin." | |||
Latest revision as of 04:01, 3 June 2025
Construction Details
bactin2 genomic sequence was derived from a 5.3 kb clone (gift of Ken Poss), which was derived in turn from a 10 kb upstream construct from Shinichi Higashijima.
p5E-bactin2 was made by PCR of the bactin2 upstream genomic sequence (using primers to add att sites), followed by a BP reaction. Upstream sequence includes 3.8 kb upstream of transcriptional start, a small 5' UTR exon, a 1.4 kb intron, and then 38 bp of 5' UTR, terminating immediately before the native start codon. Sequencing confirms that the ends of the insert are correct.
Crude Map
Screenshot from ApE showing locations of components:
299 p5E-bactin2
Download the Sequence
- Annotated Sequence (Genbank format) and Full-Length Sequence with individual component sequences included (FASTA file)
Reference
Higashijima S, Okamoto H, Ueno N, Hotta Y, Eguchi G. (1997) Dev Biol.15;192:289-99. "High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin."