PME-nlsmCherry: Difference between revisions
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show appropriate function (nuclear-localized fluorescence). | show appropriate function (nuclear-localized fluorescence). | ||
== Crude Map == | == Crude Map == | ||
Screenshot from ApE showing locations of components: | |||
:[[File:233 pME-nlsmCherrycrop.PNG|thumb|none|350px|233 pME-nlsmCherry]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:233._pME-nlsmCherry.zip|Download pME-nlsmCherry.zip]] | : [[Media:233._pME-nlsmCherry.zip|Download pME-nlsmCherry.zip]] | ||
Latest revision as of 20:33, 29 May 2025
Construction Details
pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction.
Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence).
Crude Map
Screenshot from ApE showing locations of components:
233 pME-nlsmCherry
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)