PME-mCherryCAAX**: Difference between revisions

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Created page with "== Construction Details == This template is used to display DNA sequence information in a standardized format. == Crude Map == photo showing locations of components == Sequence == * Annotated Sequence: (Genbank format) link * Full-Length Sequence with individual component sequences included: (FASTA file) link == Reference == This template is used to display DNA sequence information in a standardized format."
 
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== Construction Details ==
== Construction Details ==
This template is used to display DNA sequence information in a standardized format.
pME-mCherryCAAX was made by PCR amplification of mCherry-CAAX, using primers to add att sites, followed by a BP reaction. mCherry- CAAX is mCherry with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. The original maxiprep that was distributed (clone 232) had an H80D point mutation. Nov 2007: The new version (clone 450) has had this mutation fixed and has no remaining mutations (thanks a lot to Seok-yong Choi).


== Crude Map ==
Aug 2008: To our dismay, we realized that our current maxiprep of clone 450 again has mutation C238G, encoding the H80D amino acid change. We are currently reisolating the correct clone.
photo showing locations of components


== Sequence ==
Oct 2009: We reisolated the correct clone, now named clone 550, and have been distributing this for quite a while. Clone 450 is now obsolete.
* Annotated Sequence: (Genbank format)
link


* Full-Length Sequence with individual component sequences included: (FASTA file)
'''This clone was replaced by 550, which lacks this mutation.'''
link


 
==Download the Sequence ==
== Reference ==
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file)
This template is used to display DNA sequence information in a standardized format.
: [[Media:550.pME-mCherryCAAX.zip|Download pME-mCherryCAAX]]

Latest revision as of 20:30, 29 May 2025

Construction Details

pME-mCherryCAAX was made by PCR amplification of mCherry-CAAX, using primers to add att sites, followed by a BP reaction. mCherry- CAAX is mCherry with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. The original maxiprep that was distributed (clone 232) had an H80D point mutation. Nov 2007: The new version (clone 450) has had this mutation fixed and has no remaining mutations (thanks a lot to Seok-yong Choi).

Aug 2008: To our dismay, we realized that our current maxiprep of clone 450 again has mutation C238G, encoding the H80D amino acid change. We are currently reisolating the correct clone.

Oct 2009: We reisolated the correct clone, now named clone 550, and have been distributing this for quite a while. Clone 450 is now obsolete.

This clone was replaced by 550, which lacks this mutation.

Download the Sequence

  • Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)
Download pME-mCherryCAAX