PME-nlsEGFP: Difference between revisions
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== Crude Map == | == Crude Map == | ||
Screenshot from | Screenshot from ApE showing locations of components: | ||
:[[File:385 pME-nlsEGFPcrop.PNG|thumb|none|350px|385 pME-nlsEGFP]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:385.pME-nlsEGFP.zip|Download pME-nlsEGFP]] | : [[Media:385.pME-nlsEGFP.zip|Download pME-nlsEGFP]] | ||
Latest revision as of 20:22, 29 May 2025
Construction Details
pME-nlsEGFP was made by PCR amplification of an nls-EGFP (EGFP with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Crude Map
Screenshot from ApE showing locations of components:
385 pME-nlsEGFP
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)