PME-EGFPCAAX: Difference between revisions
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== Crude Map == | == Crude Map == | ||
Screenshot from | Screenshot from ApE showing locations of components: | ||
:[[File:384 pME-EGFPCAAXcrop.PNG|thumb|none|350px|384 pME-EGFPCAAX]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media:384.pME-EGFPCAAX.zip|Download pME-EGFPCAAX]] | : [[Media:384.pME-EGFPCAAX.zip|Download pME-EGFPCAAX]] | ||
Latest revision as of 20:20, 29 May 2025
Construction Details
pME-EGFPCAAX was made by PCR amplification of EGFP-CAAX, using primers to add att sites, followed by a BP reaction. EGFP-CAAX is EGFP with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. Sequencing confirms that all bases of the insert are correct.
Crude Map
Screenshot from ApE showing locations of components:
384 pME-EGFPCAAX
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)