P5E-hsp70l: Difference between revisions
Jump to navigation
Jump to search
Hggenusrwki (talk | contribs) Created page with "== Construction Details == This template is used to display DNA sequence information in a standardized format. == Crude Map == photo showing locations of components == Sequence == * Annotated Sequence: (Genbank format) link * Full-Length Sequence with individual component sequences included: (FASTA file) link == Reference == This template is used to display DNA sequence information in a standardized format." |
No edit summary |
||
| (3 intermediate revisions by one other user not shown) | |||
| Line 1: | Line 1: | ||
== Construction Details == | == Construction Details == | ||
p5E-''hsp70l'' was made by PCR amplification of the 1.5 kb hsp70l promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Note that hsp70l is the official ZFIN gene name for the gene originally named hsp70 by Halloran et al., 2000. Thanks to Ceri van Slyke of ZFIN for pointing this out. | |||
Sequencing shows a single base mutation in the hsp70l promoter (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock. | |||
== Crude Map == | == Crude Map == | ||
Screenshot from ApE showing locations of components: | |||
:[[File:222 p5E-hsp70.PNG|thumb|none|350px|222 p5E-hsp70]] | |||
== | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | |||
: [[Media:222.p5E-hsp70l.zip|Download p5E-''hsp70l'']] | |||
Latest revision as of 19:54, 29 May 2025
Construction Details
p5E-hsp70l was made by PCR amplification of the 1.5 kb hsp70l promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Note that hsp70l is the official ZFIN gene name for the gene originally named hsp70 by Halloran et al., 2000. Thanks to Ceri van Slyke of ZFIN for pointing this out.
Sequencing shows a single base mutation in the hsp70l promoter (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock.
Crude Map
Screenshot from ApE showing locations of components:
222 p5E-hsp70
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)