P5E-h2afx: Difference between revisions
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p5E-''h2afx'' was made by PCR amplification of 1 kb genomic DNA immediately upstream of the start codon of h2afx (NM_201073), using primers that added att sites, followed by a BP reaction. This genomic element was originally isolated by John Parant in Joe Yost's lab. Sequencing confirms that the insert is correct, although there are numerous single-base changes compared to the Sanger genomic sequence (presumably representing SNPs). | p5E-''h2afx'' was made by PCR amplification of 1 kb genomic DNA immediately upstream of the start codon of h2afx (NM_201073), using primers that added att sites, followed by a BP reaction. This genomic element was originally isolated by John Parant in Joe Yost's lab. Sequencing confirms that the insert is correct, although there are numerous single-base changes compared to the Sanger genomic sequence (presumably representing SNPs). | ||
Expression data for this gene from the Thisses [ | Expression data for this gene from the Thisses [https://zfin.org/ZDB-GENE-040426-987 ZFIN] that shows that h2afx is expressed throughout the embryo in presumptive proliferative regions. | ||
== Crude Map == | == Crude Map == | ||
Screenshot from | Screenshot from ApE showing locations of components: | ||
:[[File:380 p5E-h2afx.PNG|thumb|none|350px|380 p5E-h2afx.PNG]] | |||
==Download the Sequence == | ==Download the Sequence == | ||
* '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | * '''Annotated Sequence'''(Genbank format) and '''Full-Length Sequence with individual component sequences included'''(FASTA file) | ||
: [[Media: | : [[Media:380.p5E-h2afx.zip|Download p5E-''h2afx'']] | ||
Latest revision as of 19:54, 29 May 2025
Construction Details
p5E-h2afx was made by PCR amplification of 1 kb genomic DNA immediately upstream of the start codon of h2afx (NM_201073), using primers that added att sites, followed by a BP reaction. This genomic element was originally isolated by John Parant in Joe Yost's lab. Sequencing confirms that the insert is correct, although there are numerous single-base changes compared to the Sanger genomic sequence (presumably representing SNPs).
Expression data for this gene from the Thisses ZFIN that shows that h2afx is expressed throughout the embryo in presumptive proliferative regions.
Crude Map
Screenshot from ApE showing locations of components:
380 p5E-h2afx.PNG
Download the Sequence
- Annotated Sequence(Genbank format) and Full-Length Sequence with individual component sequences included(FASTA file)