PDestTol2CG*: Difference between revisions
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Hggenusrwki (talk | contribs) No edit summary |
Hggenusrwki (talk | contribs) No edit summary |
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: pDestTol2CG sequence | : pDestTol2CG sequence | ||
Edited 7 June 2007 to add six bases (a KpnI site that was missing). | Edited 7 June 2007 to add six bases (a KpnI site that was missing). | ||
== Crude Map == | == Crude Map == | ||
Screenshot from Sequencher showing locations of components: | Screenshot from Sequencher showing locations of components: | ||
PDestTol2CG.png | PDestTol2CG.png | ||
Latest revision as of 16:49, 13 March 2025
Construction Details
pDestTol2CG was made by PCRing the cmlc2:egfp-pA cassette, adding BglII sites in the primers, then cloning into the unique BglII site of pDestTol2pA and selecting for reverse orientation relative to the gate and existing polyA signal. The cmlc2:egfp cassette has not been sequenced, but works in functional tests.
Crudely Annotated Sequence
FASTA file with the full-length sequence as well as sequences of individual components:
- pDestTol2CG sequence
Edited 7 June 2007 to add six bases (a KpnI site that was missing).
Crude Map
Screenshot from Sequencher showing locations of components: PDestTol2CG.png